Abstract
Surrogate neutralization assays for SARS-CoV-2 that can be done without biosafety-level-3 containment and across multiple species are desirable. We evaluate a recently developed surrogate virus neutralization test (sVNT) in comparison to 90% plaque reduction neutralization tests (PRNT90) in human, canine, cat and hamster sera and found excellent concordance between the two assays. Using a panel of immune sera to other coronaviruses, we confirm the lack of cross reactivity in sVNT and PRNT90 assays.
Competing Interest Statement
The authors have declared no competing interest.
Funding Statement
Health and Medical Research Fund, the Food and Health Bureau
Author Declarations
I confirm all relevant ethical guidelines have been followed, and any necessary IRB and/or ethics committee approvals have been obtained.
Yes
The details of the IRB/oversight body that provided approval or exemption for the research described are given below:
Research Ethics Committee of the Kowloon West Cluster reference No. KW/EX-20-039 (144-27) of the Hospital Authority of Hong Kong and the Institutional Review Board of The Hong Kong University and the Hong Kong Island West Cluster of Hospitals (IRB reference number UW16-254)
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Yes
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Yes
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Yes
Data Availability
Data is stated in the manuscript